Preparation of lb/ampicillin/iptg/x-gal plates

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Preparation of cDNA fragments:. Transformation was performed according to manufacturer’s instructions onto LB/Ampicillin/IPTG/X-Gal plates.SuperFolder GFP is a highly engineered robustly folded version of GFP that. • LB growth media and plates. IPTG / X-gal screening is effective in.Chemically competent JM109 cells were transformed with 2µl of the ligation reaction and plated onto LB plates containing IPTG, X-gal, and 125µg/ml ampicillin.

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Separate on normal silica gel plates with: - a first migration with chloroform/methanol/acetic acid, 190/9/1. 2 - Preparation of ceramides from sphingomyelin.

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GFP “Fold ‘n’ Glow” Protein Solubility Assay Kit. • LB growth media and plates • Centrifuge(s). IPTG / X-gal screening is effective in the first 24.

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